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KMID : 0903619820230030230
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Journal of the Korean Society for Horticultural Science
1982 Volume.23 No. 3 p.230 ~ p.239
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In Vitro Propagation of Bulb Scale Sections of Lilium longiflorum Thunb
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Abstract
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Studies of in vitro culture were carried out to make a comparison of the responses in adventitious bulblet formation between tissues excised from different parts of bulb and cultural environments. The cultural medium used was Murashige-Skoog´s medium supplemented with various concentrations of NAA and kinetin.
The following results were obtained :
1. Callus formation from the basal part of bulb scale in the lighted environment was more effective than that in the dark. Fresh was of callus enhanced on the medium added 1.0 or 2.0 ㎎/ℓ NAA alone and 1.0㎎/ℓ NAA plus 0.3㎎/ℓ kinetin than other media.
2. Bulblet formation originated from the basal part of bulb scale increased, compared with the explants from the distal part of bulb scale in both environments. But sizes of formed bulblers in the dark were larger than those in the lighted environment. Explants from the basal part of bulb scale produced above 4 bulblets a explant on the medium added 0.5㎎/ℓ NAA alone or 0.5㎎/ℓ NAA plus 0.3㎎/ℓ kinetin in the lighted environment.
3. Continuous darkness decreased leaf number and fresh weight without regard to the distal and basal parts of bulb scale. Explants from the basal part of bulb scale increased in the lighted environment produced more and heavier leaves than those from the distal part of bulb scale.
4. Percent of root formation in the dark during early stage of cultures was higher than that in the lighted environment. But with the passage of culture time, root formation was favored in the lighted environment.
5. N and P content in the bulbets originated form the basal part of bulb scale in the lighted environment was much higher than that in the dark whereas Ca and Mg content was lower than that of the latter.
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KEYWORD
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